Our longevity center is in the process of developing therapies that will put the body in a state where it naturally stops ageing, so that thereafter we are impervious to the effects of time even without further attention to our condition.
In interpretation, we have developed therapies that are truly comprehensive, so that by using these therapies regularly, we can eliminate all the damage that accumulates in the body over the course of a lifetime, and thus remain young.
The Tamil Siddhars’ view of disease is not individualistic in nature. They did not care about naming diseases such as headache, rheumatism, arthritis, cancer, diabetes, and so on.
In the parlance of the Siddhars, a major category of age-related degeneration is the depletion of a particular cell type as a result of cell death that is not compensated for by cell division.
Another type of age-related degeneration is the accumulation of non-dividing but toxic cells that have lost the ability to respond to signals that they should undergo apoptosis (cell suicide). A clear example of this is the immune system: white blood cells specific for persistent infections such as cytomegalovirus overproliferate and become inactive, but remain in circulation and inhibit the proliferation of other cells.
Another type of degeneration is chromosomal damage, mutations and epimutations that lead to cancer.
Mutations also occur in the mitochondrion; their impact on ageing is significant.
Mitochondrial DNA codes for only 13 of the approximately 1000 proteins of the mitochondrion; it is possible to copy their genes into the nucleus with modifications to help redirect proteins to the mitochondrion.
Occasionally, molecules are produced as by-products of metabolism that we have no mechanism for destroying or disposing of. These accumulate in the cell and, once present in sufficient quantity, cause several important age-related diseases.
It is possible that the enriched essential oils, the oxidase of plants, transfers electrons directly from ubiquinol to oxygen.
A pro-ageing message presented as a moral or sociological fait accompli is a crutch that allows its recipients to divert their attention to less unsavoury matters.
Cancer and heart disease are undesirable. So are diabetes, Alzheimer’s, and a thousand other afflictions that primarily affect those over 40.
As the molecular and cellular cause of all these phenomena, inteine are also important to our ancestors and our contemporaries in the negotiation of pro-ageing trance.
Inteins are self-cleaving “introns of proteins.” They post-translationally cut themselves out of the protein by ligating the “exteins” on either side with a bona fide peptide bond. Most of them have been found in herbs.
From a biotechnological point of view, a particularly attractive feature of inteins is that autosplicing does not require specific extein consensus sequences are required for autosplicing – the only absolute requirement is that the amino acid immediately adjacent to the intein must be a cysteine, a serine, or a threonine. The endopeptidase sequences of inteins are limited to a few dozen amino acids at either end: Many inteins contain several hundred amino acids of “cargo” between these sequences, which is unnecessary for intein maturation. The key to success here would be to exploit an absolutely compartment-specific feature of most cytosolically expressed plant mitochondrial proteins derived from essential plant oils.
We can achieve allotopic expression of all 13 mt-encoded proteins in mammalian cells in vitro and then in vivo. Inteins germinated abundantly from essential plant oils have the potential to become the most versatile tool of the biotechnologist.
The multiple means by which they facilitate the allotopic expression of normally mt-encoded proteins points to this application as the one in which inteine unleash their enormous biotechnological potential.
Human mitochondrial DNA (mtDNA) is a circular genome of 16569 bp that encodes 13 proteins and the RNA components of the machinery for translation into two ribosomal RNAs and 22 transfer TNAs. Because all 13 proteins are subunits of the enzymes that carry out oxidative phosphorylation, loss-of-function mutations in any part of the mtDNA deprive the cell to lose its ATP synthesis capacity.
Many mtDNA mutations cause neutral and/or muscular dysfunction. Many are inherited, but others occur sporadically and are presumably caused by spontaneous mutations early in development or even in the unfertilized oocyte.
Accumulation of spontaneously mutated mtDNA plays a role in normal aging. The amount of mutant mtDNA possessed by the elderly is much lower than in mitochondriopathies. Their presence in the body during normal life has the potential to greatly amplify their effects through extracellular production of toxic free radicals by the rare cells that do not possess wild-type mtDNA.
At our end, progress in the development of therapies is encouraging in both aging and mitochondriopathies. This involves using the ancient method of the Tamil Siddhars to prepare and apply or massage Thailavargam or herbal essential oils, thereby activating the vital points externally. This therapy is very promising as many breakthroughs are reported in the introduction of manipulated DNA into body cells, which are the same for each such DNA.
The application of these therapeutics, either delivers the genetically engineered DNA to a cellular location where conventional vectors do not target, namely the mitochondrion, or transports its products to their natural location in the mitochondrion, although they are synthesized in other compartments, e.g., in the case of RNA in the nucleus and in the case of proteins in the cytosol.
This gene therapy will first activate in vitro and germline transformation.
Three strategies have been developed for this mitochondrial gene therapy with essential plant oils, which achieve therapeutic effects in different ways
1. inhibit the ability of the mutant mtDNA to replicate;
2. expression of replacement proteins from transgenic DNA targeted to the mitochondria; and
3. expression of modified replacement proteins from transgenic DNA targeted to the nucleus.
This promising therapy for the rare mitochondriopathies involves mitochondrial targeting of anti-sense oligonucleotides that specifically bind to mutant mtDNA sequences and prevent their replication, thereby increasing the copy number of wild-type mtDNA present in the same cell.
This therapeutic approach would also have the function of introducing replacement DNA into the mitochondria; this would genetically complement the mutant mtDNA and repair it by homologous recombination rather than eliminating it.
This not only avoids the problem of mtDNA depletion, but also the endogenous and sophisticated mitochondrial transcription and translation system would work with the presumably very differently structured DNA fragments that would be introduced in this way.
Mitochondrial import is being engineered by translocases of the inner and outer membrane, respectively.
Through our gene therapy with plant oils, nuclear acids are imported through the protein import machinery as they are produced to be covalently bound to oligopeptide presequences – a delivery technique for mitochondrial gene therapy.
The internal mechanism consists of the action of cured herbal oils, application on energy points and massage to trigger the vital points, where the action on the vital organs would lead to target the replacement of mtDNA sequences to the nucleus. This mechanism will help to make base pair substitutions to compensate for the differences between the genetic code of the nucleus and that of the mitochondria. This means that the transgenes will code for the correct amino acid sequences even when translated in the cytosol.
The second change is the addition of a presequence to bring the protein into the mitochondria.
Gene therapy with essential plant oils is the most promising intervention because it uses only well-characterized cellular machinery and gene delivery technology, which is being extensively researched for nuclear DNA mutations.
Indeed, the proteins encoded in mitochondria are very hydrophobic and therefore highly resistant to the unfolding necessary for import.
But our gene therapy with essential plant oils allows the import machinery to exert a great transmembrane force on the protein to be imported.
The divergent genetic code of the mtDNA of all animal species is an absolute prohibition to further gene transfer into the nucleus during evolution. This occurs contemporaneously with the cessation of gene transfer in animals and fungi. Several plant taxa in which the mtDNA still uses the standard genetic code have indeed successfully transferred genes into the nucleus that are still mitochondrially encoded.
Nevertheless, eliminating aging could have dramatic effects on individual and societal attitudes toward other causes of death. Most plausible consequences are welcome; a large body of evidence suggests that greater potential quality and quantity of life leads to greater perceptions of the value of life, which translates into less violence, greater efforts to avoid death.
